Primer concentration

[sylvia.c.wong]

How do I correct the data if I used different concentrations of primers in my qPCR reactions? Thanks.

-S

[Mikael Kubista]

You can use different primer concentrations for different assays. That does not matter. Should the PCR efficiencies differ (as estimated from standard curves) you can correct for that in GenEx.

Good luck

[sylvia.c.wong]

Thank you so much for your help!