NAN vs 999 on imported data

Here we discuss compatibility issues with the BIOMARK from Fluidigm

Moderator: MultiD Support

NAN vs 999 on imported data

Postby Angela » Thu May 08, 2014 2:24 am

Hi,

I notice that sometimes our exported QPCR heatmap data from the Biomark fluidigm is parsed into Genex as NAN and other times it is as 999.
From what I have tried it seems that when the heatmap has any of the following they become NAN in Genex:
-a Ct 999 score and a Fail,
-a Ct # and a Fail,
-a Ct # and a Pass that I manually Fail, or
-a Ct # that has a Pass that I "No call"
What causes a sample to become a 999 in Genex?
Is there any way to set the software to differentiate the edited scores vs the softtware called ones?

Thanks,

Angela
Angela
 
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Joined: Thu May 08, 2014 1:33 am

Re: NAN vs 999 on imported data

Postby MultiDAdmin » Thu Apr 21, 2016 10:40 am

Hi

The value 999 is set by Biomark software. I think it is part of the QC test. If the QC test fails the result will be set to 999.

BR
Amin Forootan
Multid Support
MultiDAdmin
Site Admin
 
Posts: 28
Joined: Mon Jun 30, 2008 10:02 am


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