Analysis Parameter

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Analysis Parameter

Postby pmward » Mon Oct 31, 2011 5:32 pm

Some clinical labs are using a set value of Area under the (Tm) Curve to denote LLOQ to convert to qualitative results e.g.

Melting curves exhibiting peak area <0.05 are reported as <500 copies/ml.

I've been unable to locate a reference that defines and evaluates Area under the Curve in a qPCR assay. Area under the Curve relates to end point PCR so should it be used in a qPCR assay, albeit in a qualitative manner?

I would be grateful if you could give me your opinion as to the value of this parameter.
pmward
 
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